Antony and Cleopatra is a tragedy by William Shakespeare, believed to have been written sometime between 1603 and 1607. It was first printed in the First Folio of 1623. The plot is based on Thomas North's translation of Plutarch's Life of Marcus Antonius and follows the relationship between Cleopatra and Mark Antony from the time of the Parthian War to Cleopatra's suicide. The major antagonist is Octavius Caesar, one of Antony's fellow triumviri and the future first emperor of Rome. The tragedy is a Roman play characterized by swift, panoramic shifts in geographical locations and in registers, alternating between sensual, imaginative Alexandria and the more pragmatic, austere Rome.

Cleopatra is one of Shakespeare's most complex female characters: vain and histrionic, but also imbued with a tragic grandeur. These contradictory features have led to famously divided critical responses to this late tragedy.